What is the relation between Ta and Tm of the primers in PCR reactions? ? - ta temperature primer
I read a little reading online and Ta should be 5 degrees below MC?
Tm is the melting temperature of DNA take place?
What does this formula: Tm = 4 (G + C) + 2 (A + T) OC? is G, C, A, T, the number of bases in the primer?
Thank you, I need help urgently
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